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pst
PstI is a type II restriction endonuclease isolated from the Gram negative species, Providencia stuartii. It is the isoschizomer of Streptomyces albus P restriction enzyme SalPI. It cleaves DNA at the recognition sequence 5′-CTGCA/G-3′ generating fragments with 3′-cohesive termini. This cleavage yields sticky ends 4 base pairs long. PstI is catalytically active as a dimer. The two subunits are related by a 2-fold symmetry axis which in the complex with the substrate coincides with the dyad axis of the recognition sequence. It has a molecular weight of 69,500 and contains 54 positive and 41 negatively charged residues.
The PstI restriction/modification (R/M) system has two components: a restriction enzyme that cleaves foreign DNA, and a methyltransferase which protect native DNA strands via histone methylation. The combination of both provide a defense mechanism against invading viruses. The methyltransferase and endonuclease are encoded as two separate proteins and act independently. In the PstI system, the genes are encoded on opposite strands and hence must be transcribed divergently from separate promoters. The transcription initiation sites are separated by only 70 base pairs. A delay in the expression of the endonuclease relative to methylase is due to the inherent differences of the two proteins. The endonuclease is a dimer, requiring a second step for assembly, whereas the methylase is a monomer.
PstI is functionally equivalent to BsuBI. Both enzymes recognize the target sequence 5'CTGCAG. The enzyme systems have similar methyltransferases (41% amino acid identity), restriction endonucleases (46% amino acid identity), and genetic makeup (58% nucleotide identity). These observations suggest a shared evolutionary history.
When examining the preferential double strand cleavage of DNA, the restriction endonuclease PstI bind to pSM1 plasmid DNA.
Have been looking for a long shot scope and came across this pricing. These are the Gen 2 models w/ FFP, but there are others on the site. I paid $250 (25% under list).
These are the PST Gen 2 5-25 x 50 scopes (MOA & MRAD reticles)
Product Comparison - EuroOptic.com
Up for sale is a used, but in great condition optic. Vortex Viper Gen 2. 5-25x50 illuminated Mrad. Vortex Precision Matched Rings and Defender flip caps. I still have the box and accessories. I will leave the scope leveled in the rings. Had been mounted on a Tikka 6.5 Creedmoor for about 200...
I’m looking for help from the brain trust here.
I just picked up a vortex viper pst ii and I’m trying to decide which rifle to put it on. Both are good candidates.
My goal from both rifles is to be proficient at 600 yards from a solid rest with a dream of doing some 1000 yard shots. (Though...
Want to sell for 800 shipped. Throw lever not included. Open to firearm trade offers but must be willing to ship unless within reasonable distance of Klamath Falls, OR.
Add 3.5% for PayPal G&S or any additional insurance.
Hi I have a as new in box. Vortex Viper PST 6x24x50mm illumanited mil-dot. Scope. Comes with sun shade, zero stop shims butler creek lens covers. Lifetime warranty. $525 shipped PayPal gift or fee or postal money order can do a face to face in Salem
Rifle not included
***SOLD***
Great condition, illuminating reticle. Has a little rattle can residue on it! Comes with quick release mount.
Will trade for Surefire m300, 600 tan with pressure pad
$300
Vortex Viper PST II 5-25X50 FFP, EBR-2C, MOA, PST-5255
Brand new in box, never opened or mounted.
Purchased this but have since decided to just stick with Nightforce scopes. My loss is your great deal.
Price is $900. I can ship with buyer paying any shipping/delivery fees. Shipping will have to...
I have a unused Vortex PST 4x16x50.
Don't know alot about it so I will let the pictures do the talking.
I can meet up at the Safeway in King City, Oregon or the Safeway in Sherwood, Oregon.
Only asking $450.00
Very lightly used Vortex PST Gen II 5-25x50 riflescope for sale.
Front focal plane
Illuminated EBR-2C (MRAD) reticle
5-25X magnification with 50mm objective
RZR zero stop
Includes original box, all accessories (including anti-glare hood), and Vortex flip-up protective caps for eyepiece and...
Like new Vortex Viper PST 1-4x24, includes Aero Precision mount...no trades, SOLD
Like new Vortex Strikefire...no trades, SOLD
Like new Burris AR332...no trades, SOLD
Burris fastfire 3...no trades, SOLD
Nikon Prostaff 3-9x40...no trades, SOLD
PM me and we can figure out a meet spot, all...
This is a new in box Vortex Viper PST 4-16x50 First Focal Plane scope with miliradian reticule and adjustment turrets. It has never been mounted, only pulled out of the box for pictures. This is an awesome scope, with excellent clarity, and will serve someone very well. $650 takes it home. Buyer...