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pst
PstI is a type II restriction endonuclease isolated from the Gram negative species, Providencia stuartii. It is the isoschizomer of Streptomyces albus P restriction enzyme SalPI. It cleaves DNA at the recognition sequence 5′-CTGCA/G-3′ generating fragments with 3′-cohesive termini. This cleavage yields sticky ends 4 base pairs long. PstI is catalytically active as a dimer. The two subunits are related by a 2-fold symmetry axis which in the complex with the substrate coincides with the dyad axis of the recognition sequence. It has a molecular weight of 69,500 and contains 54 positive and 41 negatively charged residues.
The PstI restriction/modification (R/M) system has two components: a restriction enzyme that cleaves foreign DNA, and a methyltransferase which protect native DNA strands via histone methylation. The combination of both provide a defense mechanism against invading viruses. The methyltransferase and endonuclease are encoded as two separate proteins and act independently. In the PstI system, the genes are encoded on opposite strands and hence must be transcribed divergently from separate promoters. The transcription initiation sites are separated by only 70 base pairs. A delay in the expression of the endonuclease relative to methylase is due to the inherent differences of the two proteins. The endonuclease is a dimer, requiring a second step for assembly, whereas the methylase is a monomer.
PstI is functionally equivalent to BsuBI. Both enzymes recognize the target sequence 5'CTGCAG. The enzyme systems have similar methyltransferases (41% amino acid identity), restriction endonucleases (46% amino acid identity), and genetic makeup (58% nucleotide identity). These observations suggest a shared evolutionary history.
When examining the preferential double strand cleavage of DNA, the restriction endonuclease PstI bind to pSM1 plasmid DNA.
Like new, includes box. Great condition!
A bonus, I will include a 3" extended aluminum sunshade with the scope ($40).
Mount is not included.
No trades, will ship.
$650
votex viper pst 4-16x50 ffp Ebr-1 Moa reticule . Got this scope with a new rifle never mounted and no ring marks is as new as it gets $650 shipped 541- 404-5233
6-24x50 sfp mil/mil ebr-1 reticle
Another scope on the chopping block, in very good condition.
$old f2f at Sportsman warehouse off 185th in hillsboro
No trades
Selling my like new (not sigthed in) Vortex Viper PST 1-4 scope. Absolutely nothing wrong with it, I just don't really go shooting anymore and would rather use the money on my house. Comes with original box, and shims.
$375
Will include the Aero 30mm mount for an additional $25.
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