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frt

In genetics, Flp-FRT recombination is a site-directed recombination technology, increasingly used to manipulate an organism's DNA under controlled conditions in vivo. It is analogous to Cre-lox recombination but involves the recombination of sequences between short flippase recognition target (FRT) sites by the recombinase flippase (Flp) derived from the 2 µ plasmid of baker's yeast Saccharomyces cerevisiae.
The 34bp minimal FRT site sequence has the sequence

5'GAAGTTCCTATTCtctagaaaGtATAGGAACTTC3'for which flippase (Flp) binds to both 13-bp 5'-GAAGTTCCTATTC-3' arms flanking the 8 bp spacer, i.e. the site-specific recombination (region of crossover) in reverse orientation. FRT-mediated cleavage occurs just ahead from the asymmetric 8bp core region (5'tctagaaa3') on the top strand and behind this sequence on the bottom strand. Several variant FRT sites exist, but recombination can usually occur only between two identical FRTs but generally not among non-identical ("heterospecific") FRTs.

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Recent contents
  1. HighLine

    New: Fightlite Industries Belt Fed Upper - MLOK

      1. For Sale
    • $5999
    • Newberg
    • Oregon
    We have 1 in store FightLite Industries MCR Belt-Fed Upper Receiver Dual-Feed Upper Mlok $5,999.99 https://www.highlinefirearms.com/products/fightlite-industries-859714006354-3591
  2. D

    WOT FRT trigger new

      1. For Sale
    • $500
    • Tacoma
    • Washington
    New in box
  3. S

    Rarebreed FRT High Prices

    Just browsing around out of curiosity sake I noticed just this past week the after market prices of the triggers has been crazy high on Gun Broker. Besides the ATF issue, is there something else on the horizon I don't know about? One recently sold for $1,550 with 30 bids...
  4. J

    ATF - FRT Document

    U.S. Department of Justice Bureau of Alcohol, Tobacco, Firearms and Explosives Office of Enforcement Programs and Services Office of Field Operations Washington, DC 20226 www.atf.gov March 22, 2022 OPEN LETTER TO ALL FEDERAL FIREARMS LICENSEES The Bureau of Alcohol, Tobacco, Firearms and...
  5. danarzh

    WOT Trigger

      1. For Sale
    • $280
    • Vancouver
    • Washington
    Brand new Wide Open Trigger, haven’t fired a single shot. For those that don’t know, it allows you to shoot your AR15 at CLOSE TO full auto speed while keeping it a semi-auto trigger (one shot per trigger pull). Feel free to search WOT Trigger or FRT Trigger if you’d like a demonstration. I...
  6. moderndave

    FRT Trigger new never installed

      1. For Sale
    • $380
    • Canby
    • Oregon
    New never installed. This is the newer version. Local face to face sales only. $380
  7. jblake00

    FRT adding a semi auto mode

    This video is the only information I can find on it, as it's not listed on the Rarebreed site.
  8. Chisnewkang

    Rare breed Frt 15

      1. For Sale
    • $500
    • Woodburn
    • Oregon
    I have 2 frt-15 triggers for sale. New never used. These are a blast! 500 each face to face cash only.
  9. Illuminaughty

    NEW Rare Breed FRT + H3 Buffer

      1. For Sale
    • $425
    • Eugene
    • Oregon
    Hi kids, get ready to add some giggle to that AR! Up for sale is a BNIB Rare Breed FRT (Forced Reset Trigger) and LuthAR H3 tungsten weighted buffer. This Rare Breed FRT was purchased directly from Rare Breed in August 2021 and is the Gen 2 version. This is important , as the trigger bar issue...
  10. J

    W.O.T Force Reset Trigger NIB! (Rare Breed Trigger)

      1. For Sale
    • $350
    • Beaverton
    • Oregon
    Wide Open Trigger / Forced Reset Trigger / Better than Rare Breed Trigger! New in box. Never used. Comes with all install hardware. **Price drop** $350 OBO Cash only unless you don’t love your dog.
  11. gryghin

    National  ATF is going after FRT

    Didn't see this posted yet. "Biden Pulls a Trump Card – Rare Breed FRT-15 Trigger Now Classified as a Machine Gun" "The agency famous for once declaring a shoe string a machine gun has now targeted Florida-based company Rare Breed Triggers."...
  12. F

    Rare breed FRT trigger and H3 buffer

      1. For Sale
    • $440
    • Beaverton
    • Oregon
    Newest gen 2weeks old Works on my sbr but doesn’t like my carbine don’t feel like messing with it Buffer cost $40
  13. safrole

    Rare breed forced reset trigger.

      1. For Sale
    • $450
    • Beaverton
    • Oregon
    Bnib, bought my second one on an impulse trying to dump it now. Looking for 450$
  14. F

    Rare breed FRT trigger

      1. For Sale
      2. For Trade
    • $430
    • Hillsboro
    • Oregon
    Got this for testing doesn’t like my springs or buffer I get light primer strikes with frontier .223 So listing it here if someone wants ... Comes with an H3 recomended buffer Might like your gun more I guess $430 buffer was $35 So basically the same price as buying one in stock with the...
  15. F

    Rare breed FRT trigger and H3 buffer

      1. For Sale
    • $500
    • Beaverton
    • Oregon
    Doesn’t want to cycle right in my SBR Don’t want to mess with it FTF pick up in Beaverton if interested H3 buffer is 1 week old just to test the trigger $35 at Oregon rifle works
  16. C

    * PENDING * RARE BREED FRT-15 trigger / new in package & slightly discounted price

      1. For Sale
    • $380
    • Hillsboro
    • Oregon
    Selling my "NEW IN PACKAGE" Rare Breed FRT-15 trigger. I'm pretty sure you all know what this is so I'm not going to provide additional information other than they include (from the manufacturer) "anti-walk" trigger pins. These are $380 dollars plus $21.69 for shipping totalling $401.69 from...
  17. M

    Rare breed frt trigger

      1. For Sale
    • $450
    • Oregon City
    • Oregon
    This is their gen 2. Ran 3 mags through it. Works amazing. Unfortunately I bought a couple more guns I need to pay for. Looking for $450. Thank you.
  18. C

    RARE BREED FRT Trigger / new in package

      1. For Trade
    • Hillsboro
    • Oregon
    ** THIS IS A "WANT TO TRADE" posting ** And any comments or questions should be done by "message". If you want talk about this or RARE BREED FRT triggers there's a separate place to do so (not my post).. PLEASE AND THANK YOU Now onto the post ~ RARE BREED FRT TRIGGER New in package If you...
  19. C

    Frt 15 for trade or sale

      1. For Sale
      2. For Trade
    • $600
    • Colorado Springs
    • Other
    Brand new frt 15 for trade or sale . Trade value 600 or better
  20. shakilloatmeal

    Rare Breed FRT-15 Trigger

      1. For Sale
      2. For Trade
    • $550
    • Damascus
    • Oregon
    Got a Rare Breed firearms FRT-15 trigger for sale brand new in the packaging. $550 cash and $565 shipped/insured. Possible trades may be accepted but would need to be done face to face. only things I’m interested in would be: - scorpion mags - 9mm 147gr fmj/jhp - modlite okw 18350 - cloud...
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