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frt

In genetics, Flp-FRT recombination is a site-directed recombination technology, increasingly used to manipulate an organism's DNA under controlled conditions in vivo. It is analogous to Cre-lox recombination but involves the recombination of sequences between short flippase recognition target (FRT) sites by the recombinase flippase (Flp) derived from the 2 µ plasmid of baker's yeast Saccharomyces cerevisiae.
The 34bp minimal FRT site sequence has the sequence

5'GAAGTTCCTATTCtctagaaaGtATAGGAACTTC3'for which flippase (Flp) binds to both 13-bp 5'-GAAGTTCCTATTC-3' arms flanking the 8 bp spacer, i.e. the site-specific recombination (region of crossover) in reverse orientation. FRT-mediated cleavage occurs just ahead from the asymmetric 8bp core region (5'tctagaaa3') on the top strand and behind this sequence on the bottom strand. Several variant FRT sites exist, but recombination can usually occur only between two identical FRTs but generally not among non-identical ("heterospecific") FRTs.

View More On Wikipedia.org
Recent contents
  1. H

    Leber v2 FRT lower for MP5K

      1. For Sale
    • $315
    • Sagle
    • Idaho
    Leber 3-position FRT lower for MP5K. Recently picked up a pictogram lower so letting this one go. Works great as tested in my AP5-P. Fully assembled and ready to go. Installs In less than 2 mins with no tools needed. Includes 3 new/unused slip trips. $315 shipped / $300 FTF in Sandpoint/CDA...
  2. B

    Arc fire forced reset selector

      1. For Sale
      2. For Trade
    • $200
    • Seattle
    • Washington
    slightly tested and has a lil dirt on it but wasn’t for me. Arc fire set with the trigger shoe and detent they send in the package, I also have the lever blocker for if you intend to use it with a Geissele trigger. $200 or Trade for a tan x300 B model can be a used one no problem, or some 9mm ammo,
  3. CountryGent

    How do you think the FRT thing will shake out?

    At the present FRTs are legal. And they will likely revolutionize the industry what with all the pent up demand. Still, there are some in the industry that are skeptical that they'll remain in a legal and relatively readily available. What say you? Here to say and let the good times roll...
  4. orier

    Frt in Call of Duty

    Will a person get busted if they use a frt in Call of Duty in Oregon.
  5. ilikegunspdx

    FRT owner goes to jail after range RSO tells police he has 3d printer in his house

    What is not clear in this video (HUGE omission imo) is was the man arrested under state/local law, or federal law? He implies federal law but looks to me that's not the case from the little info we can see. Washington DC area. View: https://youtu.be/iSO0-S5Sc6c?si=1_X313cyElGZFnwE
  6. GoPackGo

    22lr rifle with threaded barrel and FRT

      1. Want to Buy
    • Seattle
    • Washington
    Title describes it - looking for a 22lr rifle with an FRT. Reach out if you have one for sale or know of where I can get one / gunsmiths who can do conversions for a 22lr MP5 or any other 22lr rifle.
  7. ilikegunspdx

    M&p 2.0 FRT trigger

    Saw that these are available to preorder. https://freedomfingertriggers.com/pre-order-freedom-finger-frt-m-p-2-0-non-select-4140-steel/
  8. C

    Freedom Finger Trigger TX22 FRT

      1. For Sale
    • $120
    • Tukwila
    • Washington
    Installed but un-used FRT. Function test is great. While the thought of the A-10 TX22 makes me uncontrollably giggle… I don’t want to wear my slide down. View: https://youtu.be/hFFTOJZrILs $120 local - Tukwila/Renton/Kent area.
  9. bigbuddha96

    WOT Trigger BNIB

      1. For Sale
      2. For Trade
    • $250
    • Happy valley
    • Oregon
    I have Brand new in box WOT trigger , Has been opened and displayed just for pictures never installed or used. I bought 2 & have decided I don't need both. Grab it while we still can!
  10. jbett98

    WOT trigger

      1. For Sale
    • $250
    • Gresham Area
    • Oregon
    I have a brand new WOT trigger I bought when they first came available. Never installed. Asking $250.00 cash If interested, let me know. No trades.
  11. Flounder

    Geissele Super Semi-Automatic (SSA) Trigger

      1. For Sale
    • $400
    • Eugene
    • Oregon
    Found this while cleaning up a bin I got on trade. Looks a very lightly used (function test?) but I do not know the history on it (sold as-is). Comes with original packaging (which looks to be chewed on a little on 1 corner). Cash only / no trades. Willing to ship (within Oregon) for...
  12. J

    New WOT frt trigger

      1. For Sale
    • $250
    • Eugene
    • Oregon
    1 new in the box WOT frt trigger. $250 local or + $10 for shipping.
  13. Ryanmiamivice

    Last round of Super Safeties before SB243 starts

      1. For Sale
    • $70
    • COTTAGE GROVE
    • Oregon
    Me again with the safeties for your AR9 and AR15 platforms only I am doing one more push of these before the September 26th deadline. I accept Venmo, Paypal and Cashapp. I prefer venmo though. As always, cash is king. +$70 Safety only (cam, lever, rounded detent) +$30 for precut triggers...
  14. diddy_oil

    Sig MPX Gen3 with Rarebreed FRT trigger

      1. For Sale
      2. For Trade
    • $2800
    • Parkdale
    • Oregon
    Great condition Sig MPXk gen3 with a Rarebreed FRT with the 3 position selector and a Frankenstein Armory trip bar installed. It will also come with a Geissele MOX trigger with the different recoil springs, post 89k MRO on Reptilia mount, Sig flip up sights, collapsing pdw brace, an 8” MI SD...
  15. No-David

    Rare Breed FRT-15

      1. For Sale
    • $650
    • Portland
    • Oregon
    I’ve got a brand new rare breed frt-15 still in the packaging for sale. Asking $750 obo. I’m not interested in trades at this time. Thanks.
  16. Bolt_308

    Deez Nuts Tactical Super Safety Kit

      1. For Sale
    • $110
    • Salem
    • Oregon
    Sold! thanks for all the interest! I have for sale a brand new, never opened, never installed Deez Nuts Tactical D2 complete flat trigger Super Safety Kit. From the website: "Everything you need to swap out your cassette style trigger for a Super Safe setup. Includes the usual super safety and...
  17. J

    Delta Team Tactical frt-3

      1. For Sale
    • $500
    • Eugene
    • Oregon
    Delta team tactical 3 position frt. I had maybe 60 rounds threw it before I took it out. $500 no trades. Face to face preferred. Will ship to non restricted states for $10
  18. ilikegunspdx

    Good video on FRT trigger in mp5 & mp5k

    Saaaaweeeeeet! View: https://youtu.be/xMnQCDTtt04?si=6FniIDKTtG8FtxOk
  19. cityrice

    Super Selektor

      1. For Sale
      2. For Trade
    • $220
    • St Helens
    • Oregon
    IYKYK Was a backup no longer needed. $220 cash $280 Trade Value PM me if interested
  20. S

    Delete delete delete

      1. For Sale
    • $00
    • Sherwood
    • Oregon
    delete
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