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ATAC-seq (Assay for Transposase-Accessible Chromatin using sequencing) is a technique used in molecular biology to assess genome-wide chromatin accessibility(1). In 2013, the technique was first described as an alternative advanced method for MNase-seq (sequencing of micrococcal nuclease sensitive sites), FAIRE-seq and DNAse-seq (1). ATAC-seq is an emerging technique that’s gaining popularity among researchers from diverse backgrounds as it aids in a fast and sensitive analysis of the epigenome compared to DNase-seq or MNase-seq (2,3,4). The applications of ATAC-seq in enhancing the functional genomics field have been explored in recent literature in hopes to understand epigenetic regulation in the context of disease development and cell differentiation. Indeed, ATAC-seq is becoming an essential tool in epigenetics and genome-regulation research and a standard part of epigenetic analysis. It has been successfully adapted to efficiently identify open chromatin and identify regulatory elements across the genome.

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