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TRNA (guanine26-N2)-dimethyltransferase (EC 2.1.1.216, Trm1p, TRM1, tRNA (m22G26)dimethyltransferase) is an enzyme with systematic name S-adenosyl-L-methionine:tRNA (guanine26-N2)-dimethyltransferase. This enzyme catalyses the following chemical reaction

2 S-adenosyl-L-methionine + guanine26 in tRNA






{\displaystyle \rightleftharpoons }
2 S-adenosyl-L-homocysteine + N2-dimethylguanine26 in tRNAThe enzyme dissociates from its tRNA substrate between the two consecutive methylation reactions.

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