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Polymerase chain reaction (PCR) is a method widely used in molecular biology to make several copies of a specific DNA segment. Using PCR, copies of DNA sequences are exponentially amplified to generate thousands to millions of more copies of that particular DNA segment. PCR is now a common and often indispensable technique used in medical laboratory and clinical laboratory research for a broad variety of applications including biomedical research and criminal forensics. PCR was developed by Kary Mullis in 1983 while he was an employee of the Cetus Corporation. He was awarded the Nobel Prize in Chemistry in 1993 (along with Michael Smith) for his work in developing the method.The vast majority of PCR methods rely on thermal cycling. Thermal cycling exposes reactants to repeated cycles of heating and cooling to permit different temperature-dependent reactions—specifically, DNA melting and enzyme-driven DNA replication. PCR employs two main reagents – primers (which are short single strand DNA fragments known as oligonucleotides that are a complementary sequence to the target DNA region) and a DNA polymerase. In the first step of PCR, the two strands of the DNA double helix are physically separated at a high temperature in a process called DNA melting. In the second step, the temperature is lowered and the primers bind to the complementary sequences of DNA. The two DNA strands then become templates for DNA polymerase to enzymatically assemble a new DNA strand from free nucleotides, the building blocks of DNA. As PCR progresses, the DNA generated is itself used as a template for replication, setting in motion a chain reaction in which the original DNA template is exponentially amplified.
Almost all PCR applications employ a heat-stable DNA polymerase, such as Taq polymerase, an enzyme originally isolated from the thermophilic bacterium Thermus aquaticus. If the polymerase used was heat-susceptible, it would denature under the high temperatures of the denaturation step. Before the use of Taq polymerase, DNA polymerase had to be manually added every cycle, which was a tedious and costly process.Applications of the technique include DNA cloning for sequencing, gene cloning and manipulation, gene mutagenesis; construction of DNA-based phylogenies, or functional analysis of genes; diagnosis and monitoring of hereditary diseases; amplification of ancient DNA; analysis of genetic fingerprints for DNA profiling (for example, in forensic science and parentage testing); and detection of pathogens in nucleic acid tests for the diagnosis of infectious diseases.
Have come to the conclusion that I just don't enjoy shooting small guns that much, and I'm big enough that on those occasions when I do carry, concealing a full size pistol just isn't that big a deal. So this little beauty is up for trade.
Details for those not familiar, this is basically a...
Unfired CZ PCR 75 BD for sale. Comes with 2 x 10 round magazines. I purchased this new last month. In perfect condition, not even handled except by the FFL to get the serial numbers. I have decided I am too much of a revolver nut and just not a semi-auto guy and I am letting this go. Looking...
Have recently developed a desire to add a silencer to the mix, but don't have any suppressor ready handguns. Also don't carry that often, and when I do, a full size gun works as well for me as the more compact PCR, so it's time for this little beauty to go.
So up for trade is a 2016 vintage CZ...
New in Box.
Please PM with questions or to make an appt.
$649.00 + $10.00 For Background.
The PCR name stands for ‘Police Czech Republic,’ the group for whom the model was originally designed. Nearly identical in size to the CZ 75 Compact, it weighs in at a full 1/4 pound...
For sale only are four great firearms from my collection.
Please no trade offers. Prices are firm. Price does not include transfer fees.
I prefer to handle the transfer at Money Market Pawn on Powell, They are honest and reasonable with transfer fees.
First on the list is a LNIB Beretta 92x...
For sale I have a CZ 75 D PCR 9mm
4 15rd Mags
5 year warranty card still
It has upgraded night sights
Looking for cash only at the moment
No trades buyer pays transfer fee
Used Olympic Arms AR15 with KX serial number, year 2000. 20 inch barrel, Vortex crossfire II 2-7x32. Front sight was removed and Matrix Arms shroud added. Asking &50 plus transfer fees. Will meet at surrounding FFL's.
Three are 14 round magazines with flat metal base plates.
One is a 16 round with rubber baseplate.
One is a 15 round with a rubber baseplate.
One is a 14 round with a rubber baseplate.
$30 each. Only for sale where legal to buyers 21 and older. Shipping is $8 for one or all.
Thinning my handgun collection.
Up for sale is a early 2000 manufacturers date CZ 75 D Compact PCR 9MM that has been custom cerakoted to resemble a Nintendo Zapper that survived the apocalypse.
This was professionally done by Lucky Sporting Goods in Banks, OR
The coating is...
Going thru my desk, I found a magazine for a gun I no longer own, and in all likelihood won't be replacing any time soon - but I know someone on here can use it. It's a 14 round CZ 75 Compact magazine, also fits the CZ P01 and PCRs (all the same basic gun) - this is a Mec Gar made magazine. $15...